بهبود آستانه تحمل به شوری سالیکورنیا (Salicornia bigelovii) در مرحله جوانه‌زنی با پیش‌تیمار جیبرلیک‌اسید در سطوح مختلف شوری آب دریا

نوع مقاله: مقاله پژوهشی

نویسندگان

1 استادیار، مرکز ملی تحقیقات شوری، سازمان تحقیقات، آموزش و ترویج کشاورزی، یزد، ایران

2 مربی پژوهش، مرکز ملی تحقیقات شوری، سازمان تحقیقات، آموزش و ترویج کشاورزی، یزد، ایران

چکیده

     این مطالعه به‌صورت دو آزمایش جداگانه در مرکز ملی تحقیقات شوری در سال 1395 انجام شد. در آزمایش اول تأثیر 12 پیش‌تیمار مختلف شامل غلظت‌های متفاوت نیترات‌پتایسم، کلریدسدیم، کلریدکلسیم و جیبرلیک‌اسید به­همراه شاهد بر جوانه‌زنی ژنوتیپ LVYUAN no.1 سالیکورنیا (Salicornia bigelovii) به‌منظور انتخاب بهترین تیمارها برای آزمایش دوم بررسی شد. در آزمایش دوم نیز تأثیر سطوح متفاوت شوری (5/12، 25، 5/37 و 50 دسی‌زیمنس برمتر) و غلظت‌های جیبرلیک‌اسید شامل 500 و 1000 میلی‌گرم در لیتر بر جوانه‌زنی این ژنوتیپ سالیکورنیا ارزیابی شد. تیمارهای شوری با استفاده از درصدهای مختلف آب خلیج‌فارس تهیه شد. نتایج آزمایش اول نشان داد که همه پیش‌تیمارها درصد جوانه‌زنی بذر را نسبت به تیمار شاهد افزایش دادند و بیشترین تأثیر مربوط به غلظت‌های 500 و 1000 میلی‌گرم در لیتر جیبرلیک‌اسید برابر 3/142 و 1/119 درصد افزایش بود. در آزمایش دوم نیز مشخص شد که تنش شوری باعث کاهش معنی‌دار درصد و سرعت جوانه‌زنی بذر شد؛ به‌طوری‌که تیمارهای شوری 5/12، 25، 5/37 و 50 دسی‌زیمنس بر متر به‌ترتیب باعث کاهش 8/11، 3/40، 3/63 و 4/81 درصدی جوانه‌زنی بذر و کاهش 3/21، 40، 3/62 و 1/70 درصدی سرعت جوانه‌زنی گردید. کاربرد جیبرلیک اسید باعث افزایش آستانه تحمل به شوری سالیکورنیا در مرحله جوانه­زنی شد. در شرایط بدون پیش‌تیمار و پیش‌تیمار بذر سالیکورنیا با غلظت‌های 500 و 1000 میلی‌گرم در لیتر، آستانه تحمل به شوری به‌ترتیب برابر با 15/4، 31/9 و 56/6 دسی‌زیمنس بر متر و آستانه کاهش 50 درصد جوانه‌زنی برابر با 36/20، 97/35 و 39/31 دسی‌زیمنس بر متر برآورد شد. به‌طورکلی، نتایج نشان داد که درصد و سرعت جوانه‌زنی بذر سالیکورنیا با استفاده از پیش‌تیمار جیبرلیک‌اسید به‌نظر می‌رسد از طریق کاهش مواد بازدارنده رشد که منجر به خواب فیزیولوژیک بذر می‌شود، بهبود یافت.

کلیدواژه‌ها


عنوان مقاله [English]

Improving salt tolerance threshold of Salicornia bigelovii at germination stage using gibberellic acid pretreatment at different levels of seawater salinity

نویسندگان [English]

  • Gholamhasan Ranjbar 1
  • Farhad Dehghani 1
  • Hadi Pirasteh Anoushe 1
  • Mohammad Hosein banakar 2
1 Assistant Professor, National Salinity Research Center, Agricultural Research, Education and Extension Organization (AREEO), Yazd, Iran
2 Research Instructor, National Salinity Research Center, Agricultural Research, Education and Extension Organization (AREEO), Yazd, Iran
چکیده [English]

   This study was conducted in two separated experiments in the National Salinity Research Center in 2016. In the first experiment, the effect of 12 priming treatments consisted of potassium nitrate, sodium chloride, calcium chloride, and GA3 with control were investigated on seed germination of Salicornia (Salicornia bigelovii var. LVYUAN no.1) in order to select the best priming for the second experiment. In the second experiment, the effects of different salinity levels (12.5, 25.0, 37.5 and 50.0 dS m-1) and GA3 concentrations including 500 and 1000 mg L-1 were also evaluated on seed germination of the Salicornia genotype. The salinity treatments were prepared by different percentage of Persian Gulf water. The results showed that all the priming treatments increased germination percentage compared to the control, and the highest effect belonged to the concentrations of 500 and 1000 mg l-1 of GA3,by 142.3% and 119.1%, respectively. It was revealed in the second experiment that salt stress significantly reduced percentage and rate of seed germination, so that salinity treatments as 12.5, 25.0, 37.5 and 50.0 dS m-1 could reduce germination percentage by 11.8%, 40.3%, 63.3% and germination rate by 81.4% and 21.3%, 40.0%, 62.3% and 70.1%, respectively. However, GA3 priming could improve the salt tolerance and compensate a part of this loss, especially at 500 mg L-1. Application of GA3 increased salt tolerance threshold of Salicornia at germination stage. In no prim and GA3-priming treatments at 500 and 1000 mg L-1, salinity tolerance thresholds were 4.15, 9.31 and 6.56 dS m-1 and the threshold of 50% reduction was 20.36, 35.97 and 31.39 dS m-1, respectively. In general, the results indicated that seed germination and rate of Salicornia were improved using GA3.

کلیدواژه‌ها [English]

  • haloculture
  • Halophyte
  • Hormone
  • salt tress
  • sea water

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