Mehrnaz Hatami; Mohammadreza Samadi; Parisa Khanizadeh
Volume 26, Issue 4 , December 2019, , Pages 918-931
Abstract
The most of medicinal plants seeds have dormancy under normal conditions, therefore, it is necessary to know the affective factors on seed dormancy and creation optimal conditions for their germination to extensive cultivation of medicinal plants. Dracocephalum kotschyi is an important Iranian endemic ...
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The most of medicinal plants seeds have dormancy under normal conditions, therefore, it is necessary to know the affective factors on seed dormancy and creation optimal conditions for their germination to extensive cultivation of medicinal plants. Dracocephalum kotschyi is an important Iranian endemic herbs which is extinting in the Lamiaceae family. This research was conducted in order to find the most effective treatment to break seed dormancy, which is one of the major problems cultivation on a large scale and rehabilitation in the natural areas. Experimental treatments including of scarification with sand paper, 95% sulfuric acid in 3 and 6 min and their combination, gibberellic acid (125, 250 and 500 μg ml-1), nitrate potassium (% 0.2 and %0.4 in 2 times) and also for moist chilling was at (5°C for 24, 48 and 72 hours) as compare to control (watering in 24 and 48 hours). The experiment was done base on the completely randomized design (CRD). The obtained results indicated that, there was a significant difference (p-value< 0.01) between in treatments of scarification with sand paper and 95% sulfuric acid on germination percentage, germination rate, vigor index, fresh and dry weight of the plumule and radicle. The results showed that sulphuric acid treatment in 3 minutes increased significantly seed germination as compare to control and other treatments. Obtained results represents that the presence of seed hard shell and high mucilage is a physical barrier and acts as a limiting factor in the germination of dragonhead by preventing the spread of embryos or by limiting the absorption of water and gas exchange. So, application of some treatments like scarification and hormonal treatment such as gibberellin could be improved the germination of dragonhead.
Reza Dehghani Bidgoli
Volume 26, Issue 4 , December 2019, , Pages 1068-1055
Abstract
To investigate the effect of seeds priming of Astragalus gossypinus Fisher. with MS and selenium nanoparticles (SeNPs) solution in early stages of germination, a factorial experiment was conducted in a completely randomized design (CRD) with four replications in the ...
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To investigate the effect of seeds priming of Astragalus gossypinus Fisher. with MS and selenium nanoparticles (SeNPs) solution in early stages of germination, a factorial experiment was conducted in a completely randomized design (CRD) with four replications in the Laboratory of Production and Duplication Center of Municipality of Kashan, in 2017. Experimental treatments consisted of priming with MS solution at 4 levels (0 as control, 0.01, 0.05 and 0.1 % w/v), and SeNPs in 4 levels (0 as control, 0.05, 0.1 and 0.2 %w/v) for 2 hours at 25 ° C. The results of the experiments indicated that MS, SeNPs solution and interaction of treatments were significant at the 1 % level on all studied traits, including of germination percentage, radicle length, plumule length, germination coefficient, relative water content (RWC), chlorophyll a, b and total chlorophyll content. The highest germination percentage, the content of chlorophyll a and b, and plumule length were obtained in application of combined MS solution 0.01% w/v with 0.2% w/v of SeNPs. On the other hand, application of these treatments alone had positive and significant effects on the studied traits. The use of priming methods, such as used methods in this research, and its effects on Astragalus gossypinus are innovations of this research.
Maryam Makkizadeh Tafti; Ruzbeh Farhoudi; Mohammad Rastifar; Kamal Asilan
Volume 18, Issue 4 , September 2012, , Pages 569-577
Abstract
This study was conducted to study the effect of seed dormancy breaking on seed germination of Caper (Capparis spinosa L.), in a completely randomized design with four replications. Treatments of seed dormancy breaking in Caper included: untreated seeds (control), scarification with concentrated sulphuric ...
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This study was conducted to study the effect of seed dormancy breaking on seed germination of Caper (Capparis spinosa L.), in a completely randomized design with four replications. Treatments of seed dormancy breaking in Caper included: untreated seeds (control), scarification with concentrated sulphuric acid for 20 minutes, soaking in gibberellic acid (500 and 1000 ppm), Potassium Nitrate (0.3%), leaching for 12 hours and combined treatment (leaching for 12 hours and soaking in gibberellic acid (500 and 1000 ppm), leaching for 12 hours and soaking in Potassium Nitrate and scarification with sulphuric acid and soaking in gibberellic acid (500 and 1000 ppm). The results showed significant differences among seed dormancy-breaking methods. According to the results, the highest seed germination percentage of caper was observed in combined treatment (leaching for 12 hours and soaking in gibberellic acid 1000 ppm (98%) and leaching for 12 hours and soaking in gibberellic acid 500 ppm (75%). Our results showed that the treatment of seed leaching for 12 hours reduced the formation of mucilage around the seed and caused an increase in seed germination. Moreover, application of gibberellic acid or Potassium Nitrate could be just useful when the mucilage concentration of the seed coat is reduced by leaching.
Mohammad bagher Moshtaghyan; Hamid reza Keshtkar; Masood Esmaeili Sharif; Mahdi Razavi
Volume 16, Issue 1 , December 2009, , Pages 79-84
Abstract
Astragalus cyclophyllon is a perennial herb, a member of the family Papilionaceae, with vegetative form that is palatable for light animals. This experiment was done to investigate the best planting method for establishment of Astragalus cyclophyllon in Shahid Hamzavi station in Semirom. The study was ...
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Astragalus cyclophyllon is a perennial herb, a member of the family Papilionaceae, with vegetative form that is palatable for light animals. This experiment was done to investigate the best planting method for establishment of Astragalus cyclophyllon in Shahid Hamzavi station in Semirom. The study was carried out at two experiments. In first experiment, seed dormancy was broken using scarification. The results showed that stratification had the highest effect on seeds germination. In second experiment, seeds were planted based on a randomized complete block design in the spring. The methods of seed planting were pitting, furrow and surface planting. Some characteristics including percentage of plant emergence and number of simple and compound leaves were measured in the spring. There were significant differences among three methods in view point of understudy characteristics. Maximum percentage of plant emergence was related to pitting planting with 58.2% and minimum one for surface planting with 38.3%. On the basis of these results, pitting method is better than others for establishment of this plant.
Mohammad Jankju-Borzelabad; Mahdeyeh Tavakkoli
Volume 15, Issue 2 , January 2008, , Pages 215-226
Abstract
Due to the increasing attentions on multiple uses from rangelands, methods of improving seed germination rates were investigated on 10 arid rangeland species, which have ornamental and/or pharmacological values. 9 dormancy breaking treatments were applied, in a completely randomized design. Seeds ...
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Due to the increasing attentions on multiple uses from rangelands, methods of improving seed germination rates were investigated on 10 arid rangeland species, which have ornamental and/or pharmacological values. 9 dormancy breaking treatments were applied, in a completely randomized design. Seeds were then located in an incubator and daily germination rates were recorded. Between treatments, gibberlic acid caused the greatest influence, on germination of 5 out of 10 species. Low temperature, potassium nitrate, high temperature, sulphuric acid, and water imbibitions also increased germination rates of 3, 3, 3, 2, and 1 species respectively. Lowest effect was found for polyethylene glycol and wet sand treatments. The applied treatments led to high increases in germination rates of Stachys inflata, Pteropyrum aucheri, Capparis spinosa and Fortynia bungei. However, the results for Atraphaxis spinosa, Calligonum polygonoides, Calligonum bungei, Isatis cappadocica, Glaicium oxylobum, and Iris songarica may not be applicable for field workers, because of low (0-5 %) germination rate of these species, while they are valuable for researchers. Further interesting results of this experiment was a logical relationship between treatment that caused the highest increase in germination rate, and phenology stages, habitat conditions of some species.