Document Type : Research Paper
Authors
1 Ph.D Student, Department of Soil Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
2 Associate professor, Department of Soil Conservation and Watershed Management, Agricultural and Natural Resources Research Center of Kermanshah, AREEO, Kermanshah, Iran
3 Professor, Department of Soil Science, Science and Research Branch, Islamic Azad University, Tehran, Iran
4 Associate Professor, Associate professor, Soil and Water Research Institute, AREEO, Tehran, Iran
Abstract
Soil pollution by petroleum hydrocarbon pollutants is one of the most important environmental problems in different parts of the world. In the oil-rich regions of the west of the country and recent years, the depreciation of the oil extraction and exploitation system has led to leakage and distribution of oil pollutants in the soil and biological resources of the region. This study aimed to investigate the potential use of native rangeland plants and the addition of bacteria, and the amount of appropriate fertilizer to reduce pollution of total petroleum hydrocarbons (TPHs) in the soil. The pot experiment was performed in the form of a factorial design with a completely randomized design (CRD) with four replications. Plant treatments included three native or compatible plants of Medicago sativa, Agropyron trichophorum, and Atriplex canescens and bacterial treatments included Bacillus pumilus (B1), Pseudomonas putida (B2), and the combined use of these two bacteria with fertilizer recommendation based on soil testing. Also, control treatment (no plant culture, no bacterial inoculation, and no fertilization) was applied. The results showed that Medicago sativa, with a 55.16% reduction in soil TPHs had better performance than other plants. Inoculation of B1 bacterium in interaction with Medicago sativa and Agropyron trichophorum by decomposing 56.19% of soil TPHs was more successful than B2. However, Atriplex canescens was more successful than B1 in interacting with B2 bacteria and decomposing 54.64% of soil TPHs. With full fertilizer recommendation, Medicago sativa and Atriplex canescens plants were most effective, with a 68.56% reduction in soil TPHs. In soil treatment without plant cultivation, B2 bacterium had the best performance along with fertilizer recommendation. Bacteria improve phytoremediation efficiency by improving metabolic activities and developing the root system, and ultimately increasing plant biomass. The results showed a significant effect of interaction between bacteria and plant type on plant shoot dry weight at the level of α = 0.01.
Keywords
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