بررسی اثر نانو ذره سلنیوم بر جوانه‌زنی و برخی ویژگی‌های مورفوفیزیولوژیکی گون پنبه ای (Astragalus gossypinus Fisher.) در محیط کشت MS

نوع مقاله: مقاله پژوهشی

نویسنده

استادیار، گروه مرتع و آبخیزداری، دانشکده منابع طبیعی و علوم زمین، دانشگاه کاشان، ایران

چکیده

به منظور بررسی اثر پیش تیمار بذر گون پنبه ای با محلول MS و نانوذره سلنیوم در مراحل اولیه جوانه زنی، آزمایشی به صورت فاکتوریل در قالب طرح کاملا تصادفی در 4 تکرار در آزمایشگاه  مرکز تولید و تکثیر شهرداری کاشان در سال 1397 اجرا شد. تیمار های آزمایش شامل پرایمینگ با محلول MS در 4 سطح (صفر به عنوان شاهد، 01/0، 05/0 و 1/0 درصد وزنی-حجمی) و نانوذره سلنیوم در 4 سطح (صفر به عنوان شاهد، 05/0 ، 1/0 و 2/0 درصد وزنی-حجمی) به مدت 2 ساعت در دمای 25 درجه سانتی گراد بودند. نتایج آزمایشات نشان داد محلول MS ، نانوذره سلنیوم و اثر متقابل تیمارها در سطح احتمال 1% بر تمامی صفات مورد مطالعه شامل درصد جوانه­زنی، طول ریشه­چه، طول ساقه­چه، ضریب جوانه­زنی، محتوای نسبی آب، محتوای کلروفیل a،b و کلروفیل کل معنی دار بود. بالاترین میزان درصد جوانه­زنی، محتوای کلروفیل a،b و طول ساقه­چه با اعمال تیمار 01/0 درصد وزنی-حجمی محلول MS به همراه تیمار 2/0 درصد وزنی-حجمی نیترات پتاسیم بدست آمد. همچنین اعمال تیمارهای ذکر شده به تنهایی نیز بر صفات مورد مطالعه اثرات مثبت و معنی دار داشتند. استفاده از روشهای پرایمینگ از جمله روشهای مورد استفاده در این پژوهش و تأثیر آن بر روی گیاه ارزشمند گون پنبه ای از موارد نوآوری­ پژوهش حاضر می­باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Effect of Selenium Nanoparticles (Se NPs), in the Germination and Some Morphophysiological Characteristics of (Astragalus gossypinus Fisher) in MS Culture Medium

نویسنده [English]

  • Reza Dehghani Bidgoli
Assistant Professor, Department of Range and Watershed Management, University of Kashan, Iran
چکیده [English]

      To investigate the effect of seeds priming of Astragalus gossypinus Fisher. with MS and selenium nanoparticles (SeNPs) solution in early stages of germination, a factorial experiment was conducted in a completely randomized design (CRD) with four replications in the Laboratory of Production and Duplication Center of Municipality of Kashan, in 2017. Experimental treatments consisted of priming with MS solution at 4 levels (0 as control, 0.01, 0.05 and 0.1 % w/v), and SeNPs in 4 levels (0 as control, 0.05, 0.1 and 0.2 %w/v) for 2 hours at 25 ° C. The results of the experiments indicated that MS, SeNPs solution and interaction of treatments were significant at the 1 % level on all studied traits, including of germination percentage, radicle length, plumule length, germination coefficient, relative water content (RWC), chlorophyll a, b and total chlorophyll content. The highest germination percentage, the content of chlorophyll a and b, and plumule length were obtained in application of combined MS solution 0.01% w/v with 0.2% w/v of SeNPs. On the other hand, application of these treatments alone had positive and significant effects on the studied traits. The use of priming methods, such as used methods in this research, and its effects on Astragalus gossypinus are innovations of this research.

کلیدواژه‌ها [English]

  • chlorophyll
  • Astragalus
  • priming
  • RWC
  • Seed dormancy
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