بهبود صفات رویشی بذر سیاه تاغ Haloxylon ammodendron، با کاربرد پرایمینگ زیستی و مکانیکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، رشته بیابان‌زدایی، گروه مدیریت مناطق خشک، دانشکده کویرشناسی، دانشگاه سمنان، ایران

2 استادیار، گروه مدیریت مناطق خشک، دانشکده کویرشناسی، دانشگاه سمنان، ایران

3 دانشیار موسسه تحقیقات خاک و آب، سازمان تحقیقات، آموزش و ترویج کشاورزی کرج ایران.

4 دانشیار، گروه مدیریت مناطق خشک، دانشکده کویرشناسی، دانشگاه سمنان، ایران

چکیده

تاغ Haloxylon ammodendron از گونه های است که بصورت گسترده در احیای مناطق خشک مورد توجه  است. با توجه به حساسیت مرحله جوانه‌زنی بذور و مشکلات مناطق بیابانی، استقرار و زنده‌مانی گیاه وابسته به عبور از مراحل ابتدایی رویش است. هدف پژوهش حاضر، ارزیابی نقش روش‌های زیستی و مکانیکی پرایمینگ در بهبود خصوصیات رویشی نهال تاغ بود. کشت در دو بخش سینی نشا و پتریدیش درقالب طرح کامل تصادفی انجام شد. باکتری‌های Pseudomonas fluorescens ،Azotobacter chroococcum، Bacillus megaterium، Azospirillum lipoferum و Flavobacterium F40. تیمارهای پرایمینگ زیستی آزمایش بودند. دستگاه اولتراسونیک با طول موج 24 kHz به مدت 5 دقیقه تیمار پرایمینگ مکانیکی را اعمال نمود. پرایمینگ‌ زیستی و مکانیکی اختلاف معنی‌دار (01/0>p) در طول ریشه‌چه، نسبت طول ریشه‌چه به ساقه‌چه، رشد گیاهچه‌ای، وزن‌تر ریشه‌چه وساقه‌چه و وزن خشک ساقه‌چه در آزمایش پتریدیش , و سینی نشاء داشتند. تیمار اولتراسونیک سبب افزایش (33%) وزن‌تر ریشه‌چه نسبت به شاهد در سینی نشا شد. تیمار Azotobacter ، سبب افزایش معنی‌دار طول ریشه‌چه (193%)، نسبت طول ریشه‌چه به ساقه‌چه (132%)، رشد گیاهچه‌ای (108%)، وزن‌تر ریشه‌چه (232%) وزن‌تر (189%) و خشک (189%) ساقه‌چه تاغ نسبت به شاهد در پتریدیش شد. این باکتری طول ریشه‌چه (16%)، نسبت طول ریشه‌چه به ساقه‌چه (60%)، وزن‌تر (268%) و خشک (688%) برگ و سطح برگ (172%) را نسبت به شاهد در سینی نشا افزایش داد. Flavobacterium طول ساقه‌چه (40/21+) را نسبت به شاهد در سینی نشا افزایش داد. باکتری Azospirillum lipoferum وزن‌تر (65٪) و خشک (156٪) ساقه‌چه این گیاه را در سینی نشا با اختلاف معنی‌دار افزایش داد. باکتری‌های Azotobacter chroococcum، Flavobacterium وAzospirillum lipoferum برای بهبود شرایط تولید نهال تاغ قابل توصیه‌اند.

کلیدواژه‌ها


عنوان مقاله [English]

Improvement of seed vegetative traits in Haloxylon ammodendron using biological and mechanical priming

نویسندگان [English]

  • neda Ebrahimi mohamadabadi 1
  • Seyed Hassan Kaboli 2
  • Farhad Rejali 3
  • Aliasghar Zolfaghari 4
1 Ph.D. Student in Combat Desertification, Department of Arid Land Management, Faculty of Desert Studies, Semnan University, Iran
2 Assistant Professor, Department of Arid Land Management- Faculty of Desert Studies. Semnan University, Iran
3 Associate Professor, Soil & water Research Institute, Agricultural Extension and Education Karaj, Iran
4 Associate Professor, Department of Arid Land Management- Faculty of Desert Studies. Semnan University, Iran
چکیده [English]

The aim of this study was to evaluate the biological and mechanical methods of priming on improving germination characteristics and growth of Haloxylon ammodendron seedlings. Cultivation was performed in two parts of seedling tray and Petri dish in a completely randomized design in the Plant Physiology Laboratory of Semnan University. The biological treatments were Pseudomonas fluorescens, Azotobacter chroococcum, Bacillus megaterium, Azospirillum lipoferum, and Flavobacterium F-40. Ultrasonic device with 24 kHz wavelength application for 5 minutes was considered as mechanical priming treatment. Traits related to seed and seedling germination were measured. Result of analysis of variance in laboratory (Petri dish) showed significant differences for radicle length, ratio of  radicle  length to plumule  length, seedling growth, radicle and plumule fresh weight, and plumule dry weight (p<0.01). In the seedling tray, the effect of treatments was significant in radicle and plumule length, ratio of  radicle  length to plumule  length, radicle and plumule fresh weight, plumule dry weight, leaf freash and dry weight and leaf areas (p<0.01). Based on the results obtained from the comparison of means, ultrasonic treatment caused an increase (33%) in fresh weight of Haloxylon ammodendron radicle compared with the control in the seedling tray test. Azotobacter significantly increased radicle length (19%), ratio of  radicle  length to plumule  length (13%), seedling growth (10%), radicle fresh weight (23%), fresh weight (18%) and dry weight (18%) of plumule as compare to control in petri dish. In addition, Azotobacter increased radicle length (16%), ratio of  radicle  length to plumule  length (60%), leaf fresh weight (26%) and dry weight (68%), leaf area (172%) compare with the control in the seedling tray. Flavobacterium was able to increase the plumule length (21%) of Haloxylon ammodendron compared to the control in the seedling tray experiment. Azospirillum increased plumule fresh (65%) and dry weight (156%) of the plant in the seedling tray compared to the control. In general, according to the present results, the use of Azotobacter, Flavobacterium and Azospirillum bacteria was evaluated as a positive bio-priming treatment and can be used for propagation and production of Haloxylon ammodendron seedlings.

کلیدواژه‌ها [English]

  • Azotobacter chroococcum
  • Azospirillum lipoferum
  • Flavobacterium
  • Ultrasonic
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