تحقیقات مرتع و بیابان ایران

همکاری با انجمن علمی مدیریت و کنترل مناطق بیابانی ایران

شماره جاری

بر اساس شماره‌های نشریه

بر اساس نویسندگان

بر اساس موضوعات

نمایه نویسندگان

نمایه کلیدواژه ها

درباره نشریه

اهداف و چشم انداز

اعضای هیات تحریریه

اصول اخلاقی انتشار مقاله

بانک ها و نمایه نامه ها

پیوندهای مفید

پرسش‌های متداول

فرایند پذیرش مقالات

اطلاعات آماری نشریه

اخبار و اعلانات

بهبود صفات رویشی بذر سیاه تاغ Haloxylon ammodendron، با کاربرد پرایمینگ زیستی و مکانیکی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 دانشجوی دکتری، رشته بیابان‌زدایی، گروه مدیریت مناطق خشک، دانشکده کویرشناسی، دانشگاه سمنان، ایران

2 استادیار، گروه مدیریت مناطق خشک، دانشکده کویرشناسی، دانشگاه سمنان، ایران

3 دانشیار موسسه تحقیقات خاک و آب، سازمان تحقیقات، آموزش و ترویج کشاورزی کرج ایران.

4 دانشیار، گروه مدیریت مناطق خشک، دانشکده کویرشناسی، دانشگاه سمنان، ایران

چکیده

تاغ Haloxylon ammodendron از گونه های است که بصورت گسترده در احیای مناطق خشک مورد توجه  است. با توجه به حساسیت مرحله جوانه‌زنی بذور و مشکلات مناطق بیابانی، استقرار و زنده‌مانی گیاه وابسته به عبور از مراحل ابتدایی رویش است. هدف پژوهش حاضر، ارزیابی نقش روش‌های زیستی و مکانیکی پرایمینگ در بهبود خصوصیات رویشی نهال تاغ بود. کشت در دو بخش سینی نشا و پتریدیش درقالب طرح کامل تصادفی انجام شد. باکتری‌های Pseudomonas fluorescens ،Azotobacter chroococcum، Bacillus megaterium، Azospirillum lipoferum و Flavobacterium F40. تیمارهای پرایمینگ زیستی آزمایش بودند. دستگاه اولتراسونیک با طول موج 24 kHz به مدت 5 دقیقه تیمار پرایمینگ مکانیکی را اعمال نمود. پرایمینگ‌ زیستی و مکانیکی اختلاف معنی‌دار (01/0>p) در طول ریشه‌چه، نسبت طول ریشه‌چه به ساقه‌چه، رشد گیاهچه‌ای، وزن‌تر ریشه‌چه وساقه‌چه و وزن خشک ساقه‌چه در آزمایش پتریدیش , و سینی نشاء داشتند. تیمار اولتراسونیک سبب افزایش (33%) وزن‌تر ریشه‌چه نسبت به شاهد در سینی نشا شد. تیمار Azotobacter ، سبب افزایش معنی‌دار طول ریشه‌چه (193%)، نسبت طول ریشه‌چه به ساقه‌چه (132%)، رشد گیاهچه‌ای (108%)، وزن‌تر ریشه‌چه (232%) وزن‌تر (189%) و خشک (189%) ساقه‌چه تاغ نسبت به شاهد در پتریدیش شد. این باکتری طول ریشه‌چه (16%)، نسبت طول ریشه‌چه به ساقه‌چه (60%)، وزن‌تر (268%) و خشک (688%) برگ و سطح برگ (172%) را نسبت به شاهد در سینی نشا افزایش داد. Flavobacterium طول ساقه‌چه (40/21+) را نسبت به شاهد در سینی نشا افزایش داد. باکتری Azospirillum lipoferum وزن‌تر (65٪) و خشک (156٪) ساقه‌چه این گیاه را در سینی نشا با اختلاف معنی‌دار افزایش داد. باکتری‌های Azotobacter chroococcum، Flavobacterium وAzospirillum lipoferum برای بهبود شرایط تولید نهال تاغ قابل توصیه‌اند.

کلیدواژه‌ها

عنوان مقاله [English]

Improvement of seed vegetative traits in Haloxylon ammodendron using biological and mechanical priming

نویسندگان [English]

  • neda Ebrahimi mohamadabadi 1
  • Seyed Hassan Kaboli 2
  • Farhad Rejali 3
  • Aliasghar Zolfaghari 4

1 Ph.D. Student in Combat Desertification, Department of Arid Land Management, Faculty of Desert Studies, Semnan University, Iran

2 Assistant Professor, Department of Arid Land Management- Faculty of Desert Studies. Semnan University, Iran

3 Associate Professor, Soil & water Research Institute, Agricultural Extension and Education Karaj, Iran

4 Associate Professor, Department of Arid Land Management- Faculty of Desert Studies. Semnan University, Iran

چکیده [English]

The aim of this study was to evaluate the biological and mechanical methods of priming on improving germination characteristics and growth of Haloxylon ammodendron seedlings. Cultivation was performed in two parts of seedling tray and Petri dish in a completely randomized design in the Plant Physiology Laboratory of Semnan University. The biological treatments were Pseudomonas fluorescens, Azotobacter chroococcum, Bacillus megaterium, Azospirillum lipoferum, and Flavobacterium F-40. Ultrasonic device with 24 kHz wavelength application for 5 minutes was considered as mechanical priming treatment. Traits related to seed and seedling germination were measured. Result of analysis of variance in laboratory (Petri dish) showed significant differences for radicle length, ratio of  radicle  length to plumule  length, seedling growth, radicle and plumule fresh weight, and plumule dry weight (p<0.01). In the seedling tray, the effect of treatments was significant in radicle and plumule length, ratio of  radicle  length to plumule  length, radicle and plumule fresh weight, plumule dry weight, leaf freash and dry weight and leaf areas (p<0.01). Based on the results obtained from the comparison of means, ultrasonic treatment caused an increase (33%) in fresh weight of Haloxylon ammodendron radicle compared with the control in the seedling tray test. Azotobacter significantly increased radicle length (19%), ratio of  radicle  length to plumule  length (13%), seedling growth (10%), radicle fresh weight (23%), fresh weight (18%) and dry weight (18%) of plumule as compare to control in petri dish. In addition, Azotobacter increased radicle length (16%), ratio of  radicle  length to plumule  length (60%), leaf fresh weight (26%) and dry weight (68%), leaf area (172%) compare with the control in the seedling tray. Flavobacterium was able to increase the plumule length (21%) of Haloxylon ammodendron compared to the control in the seedling tray experiment. Azospirillum increased plumule fresh (65%) and dry weight (156%) of the plant in the seedling tray compared to the control. In general, according to the present results, the use of Azotobacter, Flavobacterium and Azospirillum bacteria was evaluated as a positive bio-priming treatment and can be used for propagation and production of Haloxylon ammodendron seedlings.

کلیدواژه‌ها [English]

  • Azotobacter chroococcum
  • Azospirillum lipoferum
  • Flavobacterium
  • Ultrasonic
مراجع
-  Abdel-Haleem, A. and El-Shaieny, H., 2015. Seed germination percentage and early seedling establishment of five (Vigna unguiculata L. (Walp) genotypes under salt stress. Europian Journal of Pelafia Research Library, 5(2): 22-32.
-  Abdul-Baki, A. A. and Anderson, J. D., 1973.Vigor determination in soybean seed by multiple criteria. Journal of Crop Science, 13: 630-633.
-  Alvandi, f., Dianati Tilaki, G.H.A. and Sadati, S.E., 2018. The effects of seed priming with acid ascorbic on seed germination and morphological traits of Taverniera cuneifolia under drought stress. Journal of Rangeland Science. 8(3): 264-271.
-  Amoo, S. O., Ojo, A. U. and Van Staden, J., 2008. Allelopathic potential of tetrapleura tetraptera leaf extracts on early seedling growth of five agricultural crops. South African Journal of Botany, 74: 149-152.
-  Andriamparany, J.N. and Buerkert, A., 2019. Effect of ultrasonic dormancy breaking on seed germination and seedling growth of three wild yam species (Dioscorea sp.) from SW-Madagascar. Genetic Resources and Crop Evolution, Springer, 1-8.
-  Asadi, M., 2001. Flora of Iran (Chenopodiaceae family, Beetle). Research Institute of Forests and Rangelands. 508 p.
-  Biabani, A., Zarei, M., Sancholi, S. and Romani, A., 2017. The effect of temperature and duration of the placement of seeds at different temperatures on seed germination of barley.Journal of Applied Research of Plant Ecophysiology. 4(1): 173-186.
-  Eisvand, H.R. and Latifinia, E., 2009. Effects of hydropriming and ultrasonic waves on seed leakage and germination of Artemisia (Artemisia aucheri L.). Iranian Journal of Seed Science and Research, 7(1): 1-8.
-  Eskandari, H. and AlizadehAmraie, A., 2017. Evaluation of profitability of seed priming for improvement seed germination performance of two rangeland plants (Festuca ovina and Bromus tomentellus) under drought Conditions. Journal of Rangeland Science, 7(4): 400:406.
-  Gill, P.K., Sharma, A.D., Singh, P. and Bhullar, S.S., 2003. Changes in germination, growth and soluble sugar con-tents of Sorghum bicolor (L.) Moench seeds under various abiotic stresses. Journal of Plant Growth Regulation, 40: 157-162.
-  Gutterman, Y., 1993. Seed germination of desert plants. Adaptations of Desert Organisms. Springer, Berlin, 253pp.
-  Hampton, J.G., Tekrony, D.M. and Chairperson, D., 1995. Handbook of vigour test methods. The International Seed Testing Association, Zurich, 117p.
-  Hasanzadeh Daloi, H., Omidi, H., Saedizade, A. and Alirahimi, N., 2015. The effect of seed bio-priming on germination characteristics of Shiraz Balnagoo Lallemantia royleana. 3rd National Conference on the Environment and Agricultural Research Iran.
-  Huang, Z., Zhang, X., Zheng, G. and Gutterman, Y., 2003. Influence of light, temperature, salinity and storage on seed germination on Haloxylon ammodendron. Journal of Arid Environments, 55: 453-464.
-  Jafari, M. and Tavili, A., 2013. Reclamation of Aridlands, University of Tehran press, 398pp.
-  Kavandi, A., Jafari, A. A. and Jafarzadeh, M., 2018. Effects of osmopriming on the enhancement of seed germination and seedling growth of deteriorate seeds of sainfoin (Onobrichis viciifolia) in basic and active collections of gene bank. Iranian Journal of Range and Desert Research, 25(3): 672-685.
-  Khaladbarin, A., 2015. Planting seedlings of Haloxylon. Reaserch Institiute of Forests and Rangelands Press, 205.
-  Khatibzadeh, R., Azizi, M., Aroie, H. and Farsi, M., 2013. The effect of surface disinfection and stratigraphic treatments on seed germination of Roman ginger (Levisticum officinale Koch.) In vitro conditions. Journal of Horticultural Science, 27(2): 130-138.
-  Mahdizadeh, M., Golkariyan, A., Naseri K. L. and Talebanfard A. A., 2017. Relationship between the growth of Haloxylon aphyllum and soil properties (Case study: Gonabad and Mah'velat cities). Iranian Journal of Range and Desert Research, 24(1): 30-38.
-  Movafeghi, A., Habibi, G.H. And Aliasgharpoor, M., 2008. Plant regeneration of Capparis spinosa L. using hypocotyl explants. Journal of Iran Biologhy, 21(2): 1-10.
-  Nawaz, J., Hussain, M., Jabbar, A., Nadeem, G.H.A., Sajid, M., Subtain, M. and Shabbir, I., 2013. Seed Priming A Technique. International Journal of Agricultu re and Crop Sciences, 620: 1373-1381.
-  Piri, R., Moradi, A., Balouchi, H. and Salehi, A., 2019. Improvement of cumin (Cuminum cyminum) seed performance underdrought stress by seed coating and biopriming. Journal of Scientia Horticulturae, 257: 1-8.
-  Radnejad, H., Behtarri, B., Naghipour Borj, A.A. and Haj Agha Memar, S.H., 2017. Effects of seed biopriming with Azospirillum and Azotobacter on performance and drought resistance in Festuca arundinacea Schreb. Iranian Journal of Rangeland and Desert Research. 23 (4):760-771.
-  Ramteke, A.A., Meshram, U.P. and Yaul, A.R., 2015. Effect of ultrasonic waves on seed germination of lycopersicon esculentum and anethum graveolens. International Journal of Chemical and Physical Sciences, 4: 333-336.
-  Ruttanaruangboworn, A., Chanprasert, W., Tobunluepop, P. and Onwimol, D., 2017. Effect of seed priming with different concentrations of potassium nitrate on the pattern of seed imbibition and germination of rice (Oryza sativa L.). Journal of integrative Agricalture, 16(3): 605-613.
-  Sabit, H., 1994. Forests, trees and shrubs of Iran, Yazd University Press.886 p.
-  Schelin, M., Tigabu, M., Eriksson, I., Swadago, L. and Oden, P.C., 2003. Effect of scarification, gibberllic acid and dry heat treatments on the germination of Balanties Egyptian seed from the Sudanian savanna in Burkina Faso. Journal of Seed Science Technology, 31: 605–617.
-  Seyed Sharifi, R. and Khavazi, K., 2011. Effects of seed priming with Plant Growth Promoting Rhizobacteria (PGPR) on yield and yield attribute of maize (Zea mays L.) hybrids. Journal of Food, Agriculture & Environment, 9(3): 496-500.
-  Seyed Sharifi, R. and Kavazi, K., 2012. Effect of plant growth promoting bacteria on the components of germination and seedling growth of maize (Zea mays L.). Journal of Agroecology, 3(4): 506-513.
-  Singh, J.S., Pandey, V.C. and Singh, D.P., 2011. Efficient soil microorganisms: a new dimension for sustainable agriculture and environmental development. Journal of Agriculture, Ecosystems and Environment, 140:339–353.
-  Suo, Z., Jia, Z., Lu, Q., Pan, B., Jin, Xiaobai., Xu, G., Peng, Xi., Sun, H. and Tao, Y., 2012. Distinguishing Haloxylon persicum and H. ammodendron (Haloxylon Bunge, Amaranthaceae) using DNA Marker. AASRI Conference on Computational Intelligence and Bioinformatics, 1:305-310.
-  Udayashankar, A.C., Nayaka, S.C., Reddy, M.S. and Srinivas, C., 2011. Plant growth-promoting rhizobacteria mediate induced systemic resistance in rice against bacterial leaf blight caused by Xanthomonas oryzae pv. Oryzae. Biological Control, 59(2): 114-122.‏
-  Venâncio, R. and Matins, R., 2019. Overcoming dormancy of Senna multijuga seeds with an ultrasonic probe the comparison with ultrasound and sulfuric acid baths. Journal of Forestry science, 1-7.
-  Yan, M., 2015. Seed priming stimulate germination and early seedling growth of Chinese cabbage under drought stress. South African Journal of Botany, 99: 88-92.
Zulueta-Rodriguez, R., Hernandez_Montiel, G.L., Murillo-Amador, B., Rueda-Puente, E.O., Lara Capistran, L., Troyo-Dieguez, E. and Cordoba-Matson, M.V., 2015. Effect of hydropriming and biopriming on seed germination and growth of two Mexican Fir Tree Species in danger of extinction. Journal of Forests, 6: 3109-3122.
تحقیقات مرتع و بیابان ایران
دوره 27، شماره 2
شهریور 1399
صفحه 347-356
فایل ها
هم رسانی
ارجاع به این مقاله
آمار